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Most of the recently digitised manuscripts from the National Library of the Czech Republic come from the Czech lands in the Middle Ages. In terms of content, the collection is varied, with a larger share of grammar texts and textbooks and their sets (V.H.1, V.H.8, V.H.10, V.H.15, V.H.21, V.H.24, V.H.28, V.H.32) as well as philosophical texts and commentaries used in the instruction at the Faculty of Arts of the university of Prague, both in the period before the Hussite wars and during the major resumption of its activities around the middle of the 15th century (V.H.5, V.H.9, V.H.14, V.H.18, V.H.22, V.H.30). Other manuscripts contain, for example, works of ecclesiastical law (the collection of decretals Liber Extra in V.H.34; Summa de casibus conscientiae, called Summa Pisana, in VI.A.13b), Biblical exegeses (a part of the Postilla litteralis of Nicholas of Lyra in VI.A.1; an interpretation of the Gospel of Luke in VI.A.16; a commentary by John of Wales on the Book of Revelation in VI.B.18) and collections of patristic homilies (VI.A.3, VI.B.8) and of high medieval sermons (including i.a. texts by Matthew of Cracow in VI.A.8, by Bertold of Regensburg in VI.A.20, by Jacobus de Voragine in VI.B.1, Conrad of Brundelsheim in VI.C.8 and others).

In 2021, the National Library of the Czech Republic – Slavonic Library provided access to six manuscripts and printed books from the 17th–19th centuries from its collections. The printed books are represented by the edition of the Kyiv-Pechersk Paterik (a collection of texts about the monks of the Kyiv-Pechersk Lavra) from 1661. The manuscripts comprise texts in Croatian (the play Akile/Achilles by the Croatian playwright Junije Palmotić and a poem by Petar Kanavelić written on the occasion of the mission of the ambassador of Dubrovnik to Constantinople) and in Church Slavonic (a collection of various works, A 2, as well as liturgical and paraliturgical texts).

In 2021, the Czech Pharmaceutical Museum in Kuks (a centre of Charles University – the Faculty of Pharmacy in Hradec Králové) provided access to two manuscripts from the end of the 17th century from its collections. The earlier of them contains records of the pharmacists from the Rajhrad monastery from 1676–1685 (shelf mark HK-SR-4); the other is a recipe book of Georg Philipp Parth, originating in the area of present-day Germany and probably created in 1697 (HK-SR-3).

The National Library of Medicine in Prague digitised in 2021 three documents, including one manuscript and two printed books. The manuscript (shelf mark T 468) was copied in 1819 and contains a collection of recipes and advice on the treatment of specific diseases, bloodletting, the effects of particular medical preparations, etc. The collection Scriptorum Latinorum de aneurysmatibus collectio (T 139), edited by Thomas Lauth, was printed in Strasbourg in 1785, whereas Dissertatio inauguralis medica sistens tentamen historiae medicinae by František Sazyma (D 312) comes from the Prague printing workshop of František Jeřábek from 1814.